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© Copyright Field Energy LLC. 2018    all rights reserved.

SPAGS and CLAGS (Gamma Irradiated Sterile Bioreactors)

Our patent-pending 500 liter SPAGS and 20 liter CLAGS are sterile bioreactors with TPE tube connections that are pre-sealed and gamma-irradiated. A proprietary air sparger provides a micro or millimeter bubble column through the bioreactor to supply oxygen. The supply of high volume oxygen to the biomass enables high density culture yields. We measure and optimize the Oxygen Transfer Coefficient.

Biological Filteration

Ensures all incoming fluids including water and nutrients are bacteria free. This biological filter is the essential production element. It features a triple stage hollow fiber 0.05 micron kross flow filtration system that is effective from bench top test bed to plant operations.

Sterile Tube Welder

A VanteBio® Sterile Tube Welder ensures the connection of TPE (thermo plastic elastomer) tubings without exposing interior fluid to the environment. There is no need for expensive single use sterile connectors. The connections are permanent. The inside of all tubes are completely sterile pre- and post- installation. All tubing ends are sealed prior to being gamma irradiated.

Vaporized Hydrogen Peroxide Cold Sterilization

Cold Sterilization is a must for heat sensitive PVC plumbing.  Using minimum 6-log bioburden reduction eliminates all biological contamination.

Monitoring biomass conditions in real time is critically important. Using these instruments, and knowing the growth parameters, helps us to develop feeding and growing regimes tailored to your algae species.

 

  • Exit gas analyzer evaluates CO2 and O2 gas content simultaneously in the exit gas: BlueSense BlueInOne   

  • Dissolved Oxygen sensor: Endress+Hauser

  • pH monitor: Endress+Hauser

  • Cell cytometry analyzer for cell health, fat content, cell size distribution:  MilliporeSigma

Spiral Plates Centrifuge for Harvesting and Dewatering

For species such as DHA rich C. cohnii, the cell wall is very fragile. The cells are prone to lysing by shear stress alone.  To dewater DHA rich biomass paste without lysing the cells, FPN uses Evodos spiral plate centrifuge technology. It is the most efficient and gentle dewatering concentrator. The continuous processing Evodos 50 is an essential tool to harvest biomass gently without lysing the cells.

Cold Temperature Drying

To preserve the nutrients in the biomass, especially so that the enzymes necessary for DHA synthesis are not denatured, low temperature processing is essential. There are several methods to achieve ambient temperature drying of the biomass:

•    Freeze Drying (Lyophilization)

•    Room temperature vacuum drying

•    Room temperature spray drying

•    Refractance window drying

We determine the optimum method based on your needs.

Post Process Water Treatment

During the post processing phase, after the biomass is separated, the effluent solution needs to be discharged.  It may contain unused nutrients. The effluent needs to be treated so that no bacteria nor any organism flourishes. We need to resterilize the solution.  We do it in such a way so that no residual chemical is also discharged. The discharged water is as clean as potable water.

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Technology

Our commercial algae plants are more competitive and more reliable than conventional bio-pharmaceutical fermentation plants.